Preparation and function of the membrane protein-reconstituted asymmetric liposomes

2014 Fiscal Year Final Research Report

• Project/Area Number: 25620138
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Kanagawa Academy of Science and Technology
• Principal Investigator: KAMIYA Koki 公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (70612315)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: リポソーム / 人工細胞モデル / 膜タンパク質 / フリップ-フロップ / 非対称膜 / ベシクル

Outline of Final Research Achievements

In this study, we investigated the flop dynamics of lipid asymmetric GV membranes in the presence of a membrane-interacted peptide. Our lipid asymmetric GVs were generated by a pulsed jet flow applied to a planar lipid bilayer. We observed that the DOPS-flop in asymmetric GV membranes was promoted in the presence of the peptides. Next, we successfully reconstituted flippases into the asymmetric GVs of phosphatidylserine. Finally, we observed the translocation of lipids from the extracellular leaflet to the cytoplasmic leaflet which was catalyzed by flippases. The asymmetric GVs will be useful for the study of elementary lipid-lipid or lipid-protein interactions.

Free Research Field

生体関連化学