Development of novel bacterial growth inhibition technology for application of microbial process control

2014 Fiscal Year Final Research Report

• Project/Area Number: 25630222
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Civil and environmental engineering
• Research Institution: Nagaoka University of Technology
• Principal Investigator: HATAMOTO Masashi 長岡技術科学大学, 工学(系)研究科(研究院), 助教 (20524185)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 16S rRNA / アンチセンス / 人工核酸 / 増殖抑制 / 微生物

Outline of Final Research Achievements

To evaluate an effect of anti-ribosome for microorganisms using LNA/DNA oligonucleotides, in vitro gene expression suppression experiments were conducted. From the experimental results, the designed LNA/DNA oligonucleotides had inhibitory effects depended on the dose concentrations, and the inhibitory effects were varied on its targeted site. So, as a next experiment pure bacterial cultures were used for inhibitory evaluations. From the results of growth inhibition experiments using E.coli strain AS19, little inhibitory effects were observed even thought higher concentration of LNA/DNA was used. Then, peptide added LNA/DNA oligonucleotides were designed and conducted the same experiment described above. The peptide added LNA/DNA oligonucleotides had a inhibitory effect in a certain extent but further research is needed to establish the microbial growth control technology for environmental engineering.

Free Research Field

環境微生物工学