High quality organelle recovery from microalgae with single cell disruption techniques

2014 Fiscal Year Final Research Report

• Project/Area Number: 25630372
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: TANAKA Tsuyoshi 東京農工大学, 工学(系)研究科(研究院), 教授 (20345333)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: オルガネラ回収 / 微細藻類 / 細胞破砕 / バイオディーゼル / 顕微ラマン分光法

Outline of Final Research Achievements

The cell disruption method to extract intact organelle from the cells, including hard plant cells, was developed in this study. The diatom species were used as model samples, and captured on the micro-cavity array, followed by addition of aspiration pressure. Another strategy for cell disruption is chemical method, such as acidic treatments. As comparing these two, an acidic treatment was demonstrated to be better way to achieve efficient and benign cell disruption. Furthermore, live imaging system for the micro-cavity array was developed, and further analysis and characterization were carried out using Raman microscope on the imaging system. These techniques can contribute to development of a quantitative and efficient cell disruption method.

Free Research Field

生物工学