2016 Fiscal Year Final Research Report
Advanced hybridoma technology for selective generation of stereospecific monoclonal antibodies to GPCRs and its application
| Project/Area Number |
25630376
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Mie University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Keywords | バイオテクノロジー / 生体分子 / 生体機能利用 / 免疫学 / 細胞・組織 |
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| Outline of Final Research Achievements |
Based on next generation of hybridoma technology, we could successfully generate stereospecific monoclonal antibodies (ssmAbs) against stress-related human CRHR1, which belongs to one of GPCRs. After Cell-ELISA positive hybridoma cells were cloned by limiting dilution method, not only IgM-typed but also IgG-typed ssmAbs were obtained. Furthermore, this new technology was applicable to selective production of ssmAbs against other GPCR. Immunofluorescence analysis demonstrated that ssmAb specifically recognized intact hCRHR1 on the cell surface.
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| Free Research Field |
抗体工学
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