Development of the PCR device driven by centrifugation assisted thermal convection

2014 Fiscal Year Final Research Report

• Project/Area Number: 25630379
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: SAITO Masato 大阪大学, 工学(系)研究科(研究院), 助教 (80457001)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: PCR / バイオチップ

Outline of Final Research Achievements

PCR-based genetic testing has found its applications in many fields such as detection of infectious diseases, food poisoning, etc. Especially, on-site rapid DNA detection is required. In this study, the centrifugation assisted thermal convection PCR was proposed. The PCR solution could be driven by thermal convection and continuously exchang high/low temperatures in a ring structured micro-channel without the use of typical syringe pump. After optimization of other conditions (chip coating, bubble prevention, temparature, flow rate, etc.), the on-chip DNA amplification was successfully achieved from 120 pg of initial DNA within only 15 min. From this result, the methods and conditions for the centrifugation assisted thermal convection PCR could be established. I hope this result will be step toward the practical use of the easy and rapid PCR device for all users.

Free Research Field

バイオチップ、バイオセンサー