in vivo cultivation: cultivation of microorganisms inhabiting in other organisms

2015 Fiscal Year Final Research Report

• Project/Area Number: 25630383
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Hiroshima University
• Principal Investigator: Aoi Yoshiteru 広島大学, サステナブル・ディベロップメント実践研究センター, 特任講師 (40386636)
• Co-Investigator(Kenkyū-buntansha): KANAMOTO TAISEI 聖マリアンナ医科大学, 医学部, 講師 (20260755)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 分離培養 / 難培養性微生物

Outline of Final Research Achievements

It is well known that extremely high diverse microorganism inhabit in the body of other living organisms such as animal, human, plant. However, most of them usually do not grow under artificial medium (unculturable). Our objective is to develop new cultivation methods (in vivo cultivation system) targeting such microorganisms inhabiting in other organisms, and show the advantage of newly designed methods compared with the conventional method. As a result, we succeeded to design and develop some of in vivo cultivation devices. We selected one of them and applied to the marine Soponge. Approximately 60 isolates were obtained from two methods (in vivo and standard agar plating), and comparatively analyzed their 16S rRNA gene. The diversity and the ratio of new species (sharing less than 97% of similarity to the cultivated species) are significantly higher in the isolates from in vivo cultivation.

Free Research Field

微生物生態学