Development of heart muscle creating tehcnique by heartbeat stress, and prospective of bio-artificial heart

2015 Fiscal Year Final Research Report

• Project/Area Number: 25630384
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Kumamoto University
• Principal Investigator: NAKASHIMA Yuta 熊本大学, 自然科学研究科, 准教授 (70574341)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 分化誘導 / 細胞力学刺激 / 細胞バイオメカニクス / BioMEMS

Outline of Final Research Achievements

This research objective is to guide the differentiation to heart cell by applying compressive stimulation. To achieve this goal, a microdevice for differentiation guidance was fabricated by the micromachining technique using photolithography and silicone molding. Also, a gel-micromachining technique was development by applying to the general micromachining technique for creating the cell adhesiveness surface and cell non-adhesiveness surface to the culture surface. The differentiation behavior of cells that receives compression stimulation from the fabricated device was observed by optical microscope. The differentiation ratio of cells that receives compression stimulation was compared with the control test. As a result, we clarified that the cell differentiation efficiency is increased by applying the compression stimulation.

Free Research Field

生物機能・バイオプロセス