2014 Fiscal Year Final Research Report
Basic research for development of artificial liver stem cell
Project/Area Number |
25640055
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory animal science
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Research Institution | Central Institute for Experimental Animals |
Principal Investigator |
SUEMIZU Hiroshi 公益財団法人実験動物中央研究所, その他部局等, 研究員 (40332209)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Keywords | 肝再生 / 肝幹細胞 / ヒト化マウス |
Outline of Final Research Achievements |
The purpose of this study is to identify the genuine liver stem cells that have the ability to reconstitute a liver, and is to create artificial cell with equivalent capabilities to liver stem cells. We established a simple method for inducing direct hyperplasia and compensatory regeneration of hepatocytes by administration of thioacetamide and ganciclovir to HSVtk transgenic mice, respectively. The future challenge is to establish a method for collecting proliferative liver cells, which appeared in both regenerative patterns. We identified 22 genes involved in growth promotion, and 181 genes involved in hepatic maturation by analyzing a cryopreserved human hepatocytes that are well engrafted in injured liver of TK-NOG mice. On the basis of these findings, we will advance in the exploration of “genuine liver stem cells” and the development of “humanized-liver mice model”.
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Free Research Field |
実験動物学
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