Chromothripsis-like pattern in cancer-cell genome after irradiation by a focused vertical micro-beam system, SPICE

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640062
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: INAZAWA Johji 東京医科歯科大学, 難治疾患研究所, 教授 (30193551)
• Co-Investigator(Renkei-kenkyūsha): INOUE Jun 東京医科歯科大学, 難治疾患研究所, 講師 (50568326) ; TANIMOTO Kosuke 東京医科歯科大学, 難治疾患研究所, 助教 (60611613)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: Chromothripsis / 染色体粉砕 / ゲノム再構成 / ゲノム不安定性 / マイクロビーム照射装置 / DNA二重鎖切断 / 口腔がん / 次世代シークエンサー

Outline of Final Research Achievements

Chromothripsis is a catastrophic cellular event in cancer, in which chromosomes undergo localized massive copy number alterations and rearrangements. However the causes and consequences of chromothripsis are almost unclear. We investigated whether chromothripsis was artificially induced by irradiation with the Single Particle Irradiation system to Cell (SPICE), a focused vertical micro-beam system designed to irradiate the nuclei of adhesive cells. Multiple chromosomal alterations involved in chromosome 7 with a complex translocation, suggesting chromothripsis to be induced in part by irradiation.

Free Research Field

分子細胞遺伝学