Study of the movement of damaged DNA

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640066
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Hiroshima University
• Principal Investigator: TASHIRO Satoshi 広島大学, 原爆放射線医科学研究所, 教授 (20243610)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 相同組換え / 細胞核構造

Outline of Final Research Achievements

In this study, we examined the movement of damaged DNA. For this purpose, we have established a method to induce DNA damage at a specific DNA sites and to visualize the region by FISH technique. We are now analyzing the numerous images obtained by these experiments.
We also found that over-expressed DNA repair protein RAD51 is accumulated at bundle like nuclear compartments. Immunofluorescence analysis revealed that RPA, a single strand binding protein, moved into the bundle like nuclear zone after induction of DNA damage. We are now preparing a manuscript including these new findings obtained by this project.

Free Research Field

放射線生物学