Establishment of breast PDX mouse models with high metastatic potential

2015 Fiscal Year Final Research Report

• Project/Area Number: 25640069
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Juntendo University
• Principal Investigator: orimo akira 順天堂大学, 医学(系)研究科(研究院), 准教授 (70275866)
• Co-Investigator(Kenkyū-buntansha): HORIMOTO Yoshiya 順天堂大学, 医学部, 准教授 (40424246)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 乳癌 / PDXｓ / 癌微小環境 / CAFｓ

Outline of Final Research Achievements

Human primary breast tumors were dissected from 10 breast cancer patients without any treatment in this study. Small tumor fragments were implanted orthotopically into NOG immunodeficient mice. As the result, one specimen generated an advanced tumor in 1st NOG mice, however, the tumor was not transplantable in 2nd NOG mice. The rest of 9 specimens were never grown in recipient mice for periods of 6 months. The previous report indicated very low rate (2.5%) of implantation of human breast tissues in immunodeficient mice, when 423 specimens of ER-positive human breast cancers were examined. As recent report also suggested the technical benefit of use of the intraductal injection of cancer cells but not the injection into mammary fad pad with estrogen treatment to increase implantation of cancer cells. Further study requires for overcoming the low implantation ratio and for developing the PDX model efficiently.

Free Research Field

腫瘍生物学