Development of methods for isolation of human monoclonal antibodies recognizing cancer stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640075
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: University of Toyama
• Principal Investigator: ISOBE Masaharu 富山大学, 大学院理工学研究部(工学), 教授 (70211050)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 大腸がん / がん幹細胞 / ヒトモノクローナル抗体

Outline of Final Research Achievements

We have conducted a large-scale isolation of human monoclonal antibodies from patients with colon cancer by using our high-throughput isolation system for monoclonal antibodies from single cells. We have selected antibody clones that were capable of binding with colon cancer cell lines. Those clones were further selected based on the reactivity against colon cancer stem cells identified by cancer stem cell positive markers including CD44v. We have identified candidate antigens for those clones by using immunoprecipitation combined with mass spectrum analysis as well as reactivity against protein arrays.

Free Research Field

分子遺伝学