Development of a novel method for comprehensive analysis of structural changes of cancer genome

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640078
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Kyushu University
• Principal Investigator: MIZUNO SHINICHI 九州大学, 先端医療イノベーションセンター, 准教授 (40569430)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ゲノム解析

Outline of Final Research Achievements

We developed a novel method for comprehensive analysis of structural changes of cancer genome. Our method is based on a mate-pair analysis, which enables us to collect information of both ends of DNA fragments from genome. In conventional mate-pair analysis, there must contain circularized DNAs derived from multiple DNA fragments and those mate-pair can be a cause of false positive data. To solve this problem we developed a novel method, which allows us to extract “right” information of mate-pairs derived exclusively from circularized DNAs of single DNA origin. By this method, we can make a comprehensive analysis of cancer genome in a highly precise manner, and we could obtain several candidate mate-pair information in ATL(adult T-cell leukemia/lymphoma) samples. This method is useful for analysis of structural changes of cancer genome and will provide us information for diagnosis and treatment of cancer.

Free Research Field

腫瘍学