Generation of large number of dendritic cells by induction of monocyte amplification

2015 Fiscal Year Final Research Report

• Project/Area Number: 25640091
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: Kumamoto University
• Principal Investigator: Senju Satoru 熊本大学, 大学院生命科学研究部, 准教授 (50274709)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 樹状細胞 / 単球 / がん治療 / ワクチン / 細胞治療

Outline of Final Research Achievements

We developed a method to expand human monocytes through lentivirus-mediated introduction of cMYC and BMI1, and we named the monocyte-derived proliferating cells, CD14-ML. CD14-ML differentiated into functional DC (CD14-ML-DC) upon addition of IL-4, resulting in the generation of a large number of DC. One drawback of this method was the extensive donor-dependent variation in proliferation efficiency. In the current study, we found that introduction of BCL2 or LYL1 along with cMYC and BMI1 was beneficial. Using the improved method, we obtained CD14-ML from all samples, regardless of whether the donors were healthy individuals or cancer patients. This improved method enables the generation of a sufficient number of DC for vaccination therapy from a small amount of peripheral blood from cancer patients. Information on T cell epitopes is not necessary in vaccination with cancer antigen-expressing CD14-ML-DC.

Free Research Field

免疫学