Development of Therapeutic Bacteria Machine Packaging with the Production System of Anti-Cancer Protein

2015 Fiscal Year Final Research Report

• Project/Area Number: 25640095
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Hidefumi Mukai 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (60570885)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: がん治療 / 遺伝子改変細菌 / 抗がんタンパク

Outline of Final Research Achievements

We successfully developed the B. choshinensis highly efficiently secreting murine tumor necrosis factor-alpha (TNF-alpha) and demonstrated its significant antitumor effects against the cultured TNF-sensitive L929 cells and a tendency to inhibit tumor growth in L929 bearing mice by intra-tumor injection. This is the original report showing the antitumor effects by engineered B. choshinensis, indicating that it is one of the potential bio-machines for synthetic biology and applied microbiology focusing on cancer therapy.

Free Research Field

ドラッグデリバリー、分子イメージング