Molecular adapter of the mouse hepatitis virus infection to utilize for oncolysis

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640096
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MATSUYAMA Shutoku 国立感染症研究所, ウイルス第三部四室, 室長 (90373399)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: MHV / CEACAM1a

Outline of Final Research Achievements

A murine coronavirus, mouse hepatitis virus (MHV), infects mouse cells and induces cell death. Our purpose of this study is to develop a molecular adaptor of MHV infection to induce oncolysis in target cells. This virus infects cells using a cell surface protein CEACAM1a. MHV is able to infect human cells when the expression plasmid of CEACAM1a was transfected. The 74 amino acid sequence of N-terminal domain in CEACAM1a is known to have a receptor function for MHV infection, and the binding ability of CEACAM1a to MHV is stable after denaturation with boiling. First, we had studied to find a minimum domain of viral receptor function in CEACAM1a, then the minimum domain was connected to the IgG, which named "MHV-adapter", to recognize the cell surface protein of human derived cell lines. In this moment, we unfortunately have not developed the functional MHV adapter, yet.

Free Research Field

腫瘍学 ウイルス学