High quality genome assembly and construction of high-resolution linkage map by sequencing and SNPs typing of haploid genome individuals

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640099
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: The University of Tokyo
• Principal Investigator: ASAKAWA Shuichi 東京大学, 大学院農学生命科学研究科, 教授 (30231872)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ゲノムシーケンシング / トラフグ / ニジマス / ブリ / 雌性発生 / ダブルハプロイド / ハプロイド / ホモ接合

Outline of Final Research Achievements

In this study, we planed to prepare individuals of double haploid / haploid using the techniques of gynogenesis in fish, and then we examined the usability of it for the genome sequencing, and construction of the linkage map produced by the SNP typing.
Genome sequencing results show that the N50 contig and scaffold from the double haploid individuals are much longer than those from normal hetero diploid individuals. These results indicate the effectiveness of the use of double-haploid/haploid individuals for de novo sequencing of diploid organisms.

Free Research Field

ゲノム科学