Development of translational regulation platform of circadian rhythms using a novel poly(A) determination method

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640100
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Kanazawa University
• Principal Investigator: TEI Hajime 金沢大学, 理工研究域自然システム学系, 教授 (00242115)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 概日リズム / 時計遺伝子 / 視交叉上核 / 翻訳制御 / Period1 / poly(A)

Outline of Final Research Achievements

In general, the length of poly (A) in eukaryotic mRNA shows a wide distribution, and at present, there is no precise and simple method to determine it. A novel method for its determination was developed with the improvement of conventional anchored RT-PCR, and designated as PACHINCO (Poly (A) Capture by Hairpin Chimeric Oligonucleotide)-RT-PCR. The new method can be applied for the comprehensive determination of poly (A) in whole cellular mRNAs with simply replacing 5' primers used for PACHINCO-RT-PR with sequences involved in the corresponding 3'UTRs of mRNA. Indeed, the method was optimized for an automatic microchip DNA electrophoresis apparatus, and the elongation of poly (A) of Per1 mRNA was identified using this system.

Free Research Field

ゲノム時間生物学