Plant genetic loci controlling rhizosphere microbial community composition

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640103
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: National Institute of Genetics
• Principal Investigator: IKEO Kazuho 国立遺伝学研究所, 生命情報研究センター, 准教授 (20249949)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ゲノム / 次世代シークエンス / メタゲノム / 16ｓDNA / 多様性 / バイオインフォマティクス / イネ

Outline of Final Research Achievements

If the root-associated microbial communities are substantially different depending on the plant genotype, it may be possible to breed crop genomes for advantageous rhizosphere composition.　　As a first step to addressing these questions we surveyed a series of plant species and cultivars spanning the Oryza genus, growing them in the same nutrient-poor soil and assessing the composition of their rhizosphere and surrounding soil bacteria and archaea using 16s rDNA sequencing. We found that differences between microbes associated with differing plant genotypes were not correlated with phylogenetic distance of the Oryza species or cultivars.
Microbial populations associated with the rhizosphere of wild rice species were less diverse than those associated with cultivars, suggesting that wild species may exert a greater influence on their rhizosphere than cultivated species, and the ability to select rhizosphere microbes may have been eroded during rice domestication.

Free Research Field

ゲノミクス