2013 Fiscal Year Final Research Report
Identification and characterization of salivary microbes that can stably colonize the intestine
| Project/Area Number |
25640105
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Medical genome science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Hidetoshi 麻布大学, 獣医学部, 教授 (70257294)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Keywords | 細菌叢 / 常在菌叢 / 唾液 |
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| Research Abstract |
We examined the bacterial composition and genes by analyzing 16S rRNA gene and metagenomic data obtained from the fecal microbiota longitudinally collected from gnotobiotic mice constructed by inoculation with human salivary microbiota. Also, we measured inflammatory T cells of the mouse intestinal lamina propria by flow cytometry. We found 20~30 bacterial species stably colonizing the mouse intestine. The metagenomic analysis revealed that these saliva-derived microbial communities were enriched with genes for carbohydrate metabolism but depleted in genes related with oxidative stress tolerance as compared with human salivary microbiota. We also observed the accumulation of colonic inflammatory T cells in several mice, and identified bacterial species that were significantly associated with the accumulation of the T cells. These data suggested that some human salivary microbes stably colonize the mouse intestine and have the ability of eliciting the host inflammatory response.
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[Journal Article] Dysbiosis of salivary microbiota in inflammatory bowel diseases and its association with oral immunological biomarkers2014
Author(s)
Said HS, Suda W, Nakagome S, Chinen H, Oshima K, Kim S, Kimura R, Iraha A, Ishida H, Fujita J, Mano S, Morita H, Dohi T, Oota H and Hattori
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Journal Title
DNA Res
Volume: 21
Pages: 15-25
DOI
Peer Reviewed
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