2013 Fiscal Year Final Research Report
Transomic analysis of adaptation induced by a synthetic gene circuit
| Project/Area Number |
25640110
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
System genome science
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
ITO Takashi 九州大学, 医学(系)研究科(研究院), 教授 (90201326)
|
|---|
| Project Period (FY) |
2013-04-01 – 2014-03-31
|
| Keywords | 人工遺伝子回路 / トランスオミクス / 適応 |
|---|
| Research Abstract |
The cell can adapt to various environmental changes and stably inherit the adapted states to the daughter cells. It has been long argued that such adaptation stems from the plasticity of gene circuits and that mutations play a role in fixation of the state in the population. We used a synthetic gene circuit to expose yeast cells to a selection pressure and found that they can stably maintain an adapted state even after its withdrawal. To address the mechanism underlying this phenomenon, we took a transomic analysis that integratively examines the layers of the genome DNA, chromatin, and RNAs, and revealed evidence for potential involvement of movable genetic elements, histone modifications and mitochondria.
|
