High-throughput screening for protein complexes recognizing codes of epigenetic modifications

2014 Fiscal Year Final Research Report

• Project/Area Number: 25640111
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: University of Fukui
• Principal Investigator: HONDA Shinji 福井大学, テニュアトラック推進本部, 助教 (90632167)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: DNAメチル化 / アカパンカビ / ヒストン修飾 / エピジェネティクス

Outline of Final Research Achievements

It has been proposing that multiple combinations of modifications of DNA and/or histones work as codes to direct various biological phenomena. Although the modifications has been known to be recognized by specific protein(s), the multiple code recognition system remains unclear. In this study, we developed a system to identify proteins associated with specific genomic domains such as centromeres which are modified using mutants lacking DNA and/or histone modifications in Neurospora. Furthermore, we found novel recognition machineries using the system, verifying the utility.

Free Research Field

エピジェンティクス