2013 Fiscal Year Final Research Report
Basic reserch of long term preservation at room temperature for mammalian somatic cells by freeze drying
| Project/Area Number |
25640121
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservation of biological resources
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| Research Institution | Kochi University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Keywords | 凍結乾燥 / 哺乳類体細胞 / 遺伝資源保存 |
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| Research Abstract |
This research carried out basic study as preservation technique of a new mammalian somatic cells by freeze-drying. In bovine fibroblast cells, total aomount of protein and the number of gamma-tubulin decreased after freeze-drying. Furthermore, mitochondrial membrane potential was not observed in the cell after freeze-drying. It was thought that addition of trehalose and epigallocatechin gallate (EGCG)in a cell culture medium was ineffective to protection of a cell membrane and organelle. However, when temperature higher than a glass transition point after FD, trehalose and EGCG could prevent damage in nuclear DNA. These result suggested that freeze-dried somatic cells has the possibility of preservation at room temperature by trehalose and EGCG .
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