2014 Fiscal Year Final Research Report
A genome-wide RNAi screening for clarifying the molecular mechanism of osmotic stress response in the mammalian cell
Project/Area Number |
25650004
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
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Research Institution | The University of Tokyo |
Principal Investigator |
NAGURO Isao 東京大学, 薬学研究科(研究院), 講師 (80401222)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
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Keywords | ASK3 / 浸透圧 / RNAiスクリーニング / MAPK / キナーゼ / ハイコンテントアナリシス |
Outline of Final Research Achievements |
We studied the molecular mechanism how mammalian cells sense and transduce osmotic stress information, using ASK3 as an original probe. According to the results obtained from genome-wide siRNA screenings for ASK3 regulators, it is suggested that hypo- and hyper-tonic stress are received by different system. Moreover, we found that an unexpected molecule is involved in the signal transduction of hypertonic stress. Our results contribute to the comprehensive understanding of osmotic stress response in the mammalian cells and blood pressure regulation, which is closely related to the osmotic stress-responsive molecules such as ASK3.
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Free Research Field |
分子生物学
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