2015 Fiscal Year Final Research Report
DNA methylation coupled with transcription elongation machinery
| Project/Area Number |
25650007
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Shoji Tajima 大阪大学, たんぱく質研究所, 教授 (50132931)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | DNAメチル化 / 転写伸長 |
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| Outline of Final Research Achievements |
Recent whole genome bisulfite sequencing analysis with next generation sequencer reveals that DNA methylation exist not only at promoter, but also at gene bodies of highly transcribed genes. Previously, I purified the Dnmt3a complex from mouse embryonic stem cell (mESC), and found that Rpb1 and Supt6h, which are components of transcriptional elongation machinery, were co-purified with Dnmt3a. To elucidate the mechanism underling in the gene body methylation, I focused in an interaction of Dnmt3a and transcriptional elongation machinery. I found that (1) Dnmt3a and Rpb1 bound in mESC, NIH3T3 and N1E115, (2) Dnmt3a localized on gene body of Atp5b gene, which is highly transcribed in mESC, in the same manner with the elongating Rpb1 (phosphorylated CTD-Ser2) and H3K36me3, (3) both of Dnmt3a and Dnmt3b contributed on the gene body methylation.
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| Free Research Field |
エピジェネティクス
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