Real-time imaging of histone modification dynamics in the specific gene loci

2015 Fiscal Year Final Research Report

• Project/Area Number: 25650009
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Tokyo Institute of Technology (2014-2015); Osaka University (2013)
• Principal Investigator: Sato Yuko 東京工業大学, 生命理工学研究科, 科学研究費研究員 (70435882)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: エピジェネティクス / イメージング / クロマチン

Outline of Final Research Achievements

This project aimed to visualize the dynamics of histone modification in the specific gene loci in living cells. To visualize specific genome loci, we searched repetitive DNA sequence enriched in single loci and designed TALE protein. However, TALEs that are expected to bind to these sequences failed to label them in living cells. On the other hand, using a mintbody specific to phosphorylated RNA polymerase II combined with ANCHOR system that labeled the specific locus, active transcription at the single gene locus was visualized in human cells.

Free Research Field

細胞生物学