Roles of proteinphosphatases in regulation of chromosome DNA replication

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650010
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: MASUKATA Hisao 大阪大学, 理学(系)研究科(研究院), 教授 (00199689)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: PP1 / PP2A / Rif1 / テロメア / 複製開始点 / 染色体 / 分裂酵母

Outline of Final Research Achievements

Chromosomal DNA replication initiates at many loci called replication origins on the chromosome, and individual replication origins are activated at specific timing in S phase. Since the process of initiation requires phosphorylations of initiation factors by protein kinase, such as cyclin-dependent kinase (CDK) and Dbf4-dependent kinase (DDK), we asked whether protein phosphatase, such as PP1 and PP2A, are involved in the control. Using the auxin-induced protein degradation system (AID) in fission yeast, we found that PP1 is essential for the control of replication timing, whereas PP2A is not required.

Free Research Field

分子遺伝学