Identification of factors involved in the eukaryotic mismatch repair reaction

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650011
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: TAKAHASHI Tatsuro 大阪大学, 理学(系)研究科(研究院), 助教 (50452420)
• Research Collaborator: KAWASOE Yoshitaka ; TERUI Riki
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ミスマッチ修復 / クロマチン / DNA損傷応答 / DNA複製 / ツメガエル / 卵抽出液 / 試験管内系 / DNA修復

Outline of Final Research Achievements

Eukaryotic chromosomal DNA is wrapped around histones to be assembled into chromatin, whose dynamic remodeling is linked to various DNA transactions. The mismatch repair system maintains DNA replication fidelity by correcting replication errors. Impairment of this system thus leads to oncogenesis in human. Here, we sought factors involved in the mismatch repair reaction in the context of chromatin, using Xenopus egg extract that promotes chromatin assembly in vitro. We have identified at least 9 factors whose involvements in mismatch repair have not previously been documented, and 3 of which were chromatin-related factors. Having identified these new factors, this work established an important step toward understanding of the mismatch repair reaction on chromatin.

Free Research Field

分子遺伝学