Novel genome-organization mechanism by telomere-binding proteins

2015 Fiscal Year Final Research Report

• Project/Area Number: 25650012
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: Kanoh Junko 大阪大学, たんぱく質研究所, 准教授 (10323809)
• Research Collaborator: HASEGAWA Yudai
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: テロメア / DNA複製 / ゲノム / 複製開始点

Outline of Final Research Achievements

Replication of chromosomal DNA starts at the replication origins at fixed timing during S phase. In this study, we investigated the effect of telomere defects in genome-wide replication timing. We observed the accelerated replication at some late origins when the telomere DNA was abnormally elongated by the Rap1 or Poz1 deletions. Furthermore, we found that the localization of PP1 phosphatase was decreased at the late origins in those mutants. Our results indicated that the telomere length maintenance is important for the normal replication timing of chromosome DNA.

Free Research Field

分子生物学、分子遺伝学