2015 Fiscal Year Final Research Report
Analysis of Rab membrane insertion mechanism using artificial membranes
Project/Area Number |
25650018
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | The University of Tokyo |
Principal Investigator |
ITO Sakurako 東京大学, 分子細胞生物学研究所, 助教 (60597152)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | Rab / 膜 / GEF |
Outline of Final Research Achievements |
In eukaryotic cells, Rab family small GTPases present the identity of each membrane fractions. Rab forms a complex with RabGDI in cytosol, and when necessary, appropriate Rab needs to be detached from RabGDI and inserted into the membrane. In this study, we have tried to reconstruct the Rab insertion system on the artificial membrane. Yip family transmembrane proteins and RabGEFs are proposed to function in the Rab insertion system. We have successfully prepared these components, which is necessary for the reconstruction of the Rab insertion system.
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Free Research Field |
X線結晶構造解析
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