Development of a new method to detect or measure lipid raft by nanoparticles and mass spectrometry.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650022
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: SENO Keiji 浜松医科大学, 医学部, 准教授 (50283908)
• Co-Investigator(Kenkyū-buntansha): YAMAHAMA Yumi 浜松医科大学, 医学部, 教務員 (90242784)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 脂質ラフト / 質量分析 / ナノ粒子 / 生体膜 / 視細胞 / ロドプシン

Outline of Final Research Achievements

In a biomembrane, there are heterogeneous lipid composition like lipid raft. These heterogeneity supports many proteins' functions. Despite the importance of this heterogeneity, there are no direct method to detect these lipid heterogeneity. In this research, we have used antibody labeled nanoparticles and mass spectrometry to develop a new method that analyze the lipids surrounding the antigen proteins. As the results, we detected fatty acids which composing phospholipids which surrounds the antigen proteins by using gold nanoparticle, antibody and antigen complex linked covalently each other.

Free Research Field

分子生理学，生物物理学