In-cell NMR study on regulatory mechanism of calcium release by ryanodine receptor

2016 Fiscal Year Final Research Report

• Project/Area Number: 25650024
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: The University of Tokushima
• Principal Investigator: MAITA Ayako 徳島大学, 大学院医歯薬学研究部(医学系), 特別研究員(RPD) (60415106)
• Research Collaborator: TSUGITA Saki 徳島大学, 大学院医歯薬学研究部
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: カルシウム恒常性 / NMR / サルコペニア

Outline of Final Research Achievements

Ryanodine receptor is the sarcoplasmic reticulum calcium release channel. We tried to investigate the interaction between ryanodine receptor and its stabilizing factor, FKBP12, by in-cell NMR. However, we did not succeed to deliver the protein of interest to myogenic cells. Thus, I changed the originally planned research project for the study focused on the interaction the protein complex (uncoupling protein 3 (UCP3) and HAX-1) that was involved in the control of the calcium influx into the mitochondria. The NMR and CD experiments showed that calcium-induced folding and stabilization of the C-terminal region of HAX-1 enable it to interact with UCP3. Moreover, the NMR experiment using two different detergents suggested that the C-terminal region could bind to biological membrane.

Free Research Field

構造生物学