2014 Fiscal Year Final Research Report
Structural biology of dioxin receptor
| Project/Area Number |
25650029
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
OHTO Umeharu 東京大学, 薬学研究科(研究院), 講師 (90451856)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | ダイオキシン / 環境汚染 |
|---|
| Outline of Final Research Achievements |
For crystallogaraphic analysis of dioxin receptor, we constructed expression system of AhR and its coreceptor ARNT. We examined various sources of AhR/ARNT genes, expression hosts, and optimum regions for expression. We established purification protocol for AhR/ARNT. We conducted DNA binding experiments and crystallization screening using purified AhR/ARNT.
|
| Free Research Field |
構造生物学
|
