2014 Fiscal Year Final Research Report
Developement of FRET-sensor for photosynthetic products
| Project/Area Number |
25650037
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 生体エネルギー変換 / 光合成 |
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| Outline of Final Research Achievements |
In the light conditions, photosynthetic systems produce NADPH and ATP in higher plants. NADPH and ATP are further used for the reduction of CO2 in the chloroplast stroma. In this study, we proposed to develop NADPH sensor by fluorescent energy transfer (FRET) using NADPH-binding protein and Green fluorescent protein (GFP)-derivatives.
We constructed the fused proteins which CFP (GFP-derivative) protein was fused at N-terminus of NADPH-binding protein and YFP (GFP-derivative) protein was fused at C-termius of NADPH-binding protein. The fusion-proteins were overexpressed.
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| Free Research Field |
植物生理学
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