Force Transmission Mechanism by Dynein Molecules That Controls Ciliary Beating Movement

2015 Fiscal Year Final Research Report

• Project/Area Number: 25650048
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: TOYOSHIMA Yoko 東京大学, 総合文化研究科, 教授 (40158043)
• Co-Investigator(Kenkyū-buntansha): YAJIMA Junichiro 東京大学, 大学院総合文化研究科, 准教授 (00453499)
• Research Collaborator: SUGAWA Mitsuhiro ; KOBAYASHI Takuya ; ICHIKAWA Muneyoshi ; YAMAGUCHI Shin ; SAITO Kei ; KOISO Yurika
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 繊毛運動 / 軸糸 / 微小管 / ダイニン / 基底小体

Outline of Final Research Achievements

We have developed an optical microscopic imaging system that is able to observe the 3-dimentional motion of individual cilia in Tetrahymena thermophile with high spatial precision, and that detected the beating pattern. The genes encoding axonemal dyneins, dynein binding proteins and basal body localized proteins fused to EGFP were successfully translated into Tetrahymna. Our results suggest that responses of ciliary movement depending on dynein activities localized to the axoneme are quantified and therefore dynein functions in a ciliary motion system can be quantified.

Free Research Field

生物物理学