Development of the microscopy for optical sectioning chemiluminescence imaging

2016 Fiscal Year Final Research Report

• Project/Area Number: 25650052
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Osaka University
• Principal Investigator: Arai Yoshiyuki 大阪大学, 産業科学研究所, 助教 (20444515)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: イメージング

Outline of Final Research Achievements

Chemiluminescence imaging enable us excitation light free imaging since the chemiluminescencent probes can emit the light by the reaction with the chemiluminescent substrates. However, three dimensional imaging cannot be done because the system do not use illumination optical system. Here I develop the microscopy by utilizing the spherical aberration of the optical lens for three dimensional chemiluminescence imaging. Although my system could not be used for the extraction of optical plane of chemiluminescence image, the system might have the possibility for other fluorescence imaging system.

Free Research Field

生物物理学