Fluorescent Ca2+ probe for high-resolution Ca2+ imaging

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650055
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Waseda University
• Principal Investigator: SUZUKI Madoka 早稲田大学, 重点領域研究機構, 准教授 (40350475)
• Co-Investigator(Kenkyū-buntansha): ARAI Satoshi 早稲田大学, 重点領域研究機構, 招聘研究員 (70454056)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 可視化 / 生物物理 / ナノ材料 / マイクロ・ナノデバイス / 細胞・組織

Outline of Final Research Achievements

The purpose of this study was to develop a novel fluorescent Ca2+ probe. In the first fiscal year (FY), we prepared new probes by using compounds newly synthesized. The microscopy setup was arranged to fit these probes. The probes were evaluated in HeLa cells under stimulus. However, in the second FY, we found that the fluorescence intensity was insufficient to obtain sufficient signal-to-noise ratio. Thus, we moved on to the next probe based on the knowledge gained in this study. We also incorporated the ability of targeting into the probe.

Free Research Field

生物物理学