A novel purification method for endogenous protein using ASKA technique

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650061
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: ICHIJO Hidenori 東京大学, 薬学研究科(研究院), 教授 (00242206)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ASK1 / 化合物プルダウン / シグナル複合体

Outline of Final Research Achievements

ASK1 forms a huge complex with various molecules to sense wide variety of stresses and induce a diverse array of physiological responses. In this study, we established novel pull-down assay system for purifying ASK1 complex from a variety of tissues/cells, and we obtained dozens of novel components of ASK1 complex. Since ASK1 has different roles in different tissues/cells, we are going to elucidate how ASK1 modulates diverse kinds of stress responses in each tissue/cell.

Free Research Field

分子生物学、生化学