2015 Fiscal Year Final Research Report
Analysis of a transcriptional network for potential pluripotency in primordial germ cells
Project/Area Number |
25650065
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Cell biology
|
Research Institution | Kyoto University |
Principal Investigator |
Kurimoto Kazuki 京都大学, 医学(系)研究科(研究院), 准教授 (20415152)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | ChIP-seq / OCT4 / 始原生殖細胞 |
Outline of Final Research Achievements |
Primordial germ cells (PGCs), precursors of sperm and oocyte, is an embryonic cell type, which potentially possess the pluripotency, a cellular ability to give rise to all of cell types in the body. The explicit pluripotency (e.g., pluripotent stem cells, mismigrate PGCs) is a harmful property, which give rise to teratoma, but the mechanism to make pluripotency to be a “potential” one is largely elusive. Recently, I developed a method to faithfully identify genomic binding sites of transcription factors in a small number of cells (~10,000 cells). In this study, I investigated application of this method to pluripotency transcription factors expressed in PGCs, and identified the OCT4 binding pattern in PGC-like cells distinct form that of ES cells, suggesting a transcription network characteristic of potential pluripotency.
|
Free Research Field |
分子生物学・ゲノム科学
|