Generation of an eukaryote harboring genes transferred from mitochondria to the nucleus

2015 Fiscal Year Final Research Report

• Project/Area Number: 25650067
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: OKAMOTO Koji 大阪大学, 生命機能研究科, 准教授 (40455217)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ミトコンドリア / ミトコンドリアDNA / 酵母 / 合成生物学

Outline of Final Research Achievements

Mitochondria possess their own genome whose oxidative stress-induced mutations are thought to cause intractable disorders such as mitochondrial disease and aging. In this study, we aimed to transfer genes from mitochondria to the nucleus and express them. Particularly, we sought to (1) change codon usage for nuclear genes, (2) optimize codon usage for efficient protein synthesis via cytosolic ribosomes, and (3) target synthesized polypeptides to mitochondria by adding a presequence.

Free Research Field

分子細胞生物学