Study of programmed cell death in mice using in vivo imaging method

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650078
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses (2014); Osaka University (2013)
• Principal Investigator: TSUJIMOTO Yoshihide 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), その他部局等, 研究所長 (70132735)
• Research Collaborator: IMAGAWA Yusuke 奈良先端科学技術, 大学院バイオサイエンス研究科, 特別研究員 (20614770) ; MATSUOKA Yosuke 大阪大学, 大学院医学系研究科, 特別研究員 (60263258)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: programmed cell death / apoptosis / necrotic cell death / autophagy / propidium iodide

Outline of Final Research Achievements

Programmed cell death (PCD) plays a crucial role in developmental morphogenesis and maintenance of tissue homeostasis in metazoans. PCD is mediated by not only apoptosis but also, as we have been proposing, non-apoptotic programmed death (NAPD) mechanism. We have developed an in vivo imaging method using propidium iodide for NAPD in mice. Using this method, we have performed quantitative and extensive study of developmental PCD in mice and identified PI-stained PCD, which was not affected by apoptosis-deficiency, indicating the occurrence of NAPD in vivo. One of them is PCD occurring during bone formation. We found that this NAPD is mediated by a process dependent on ATG9 which is involved in autophagy.

Free Research Field

分子細胞生物学