The identification of lactic acid bacteria derived reprogramming factor(s).

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650082
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: OHTA KUNIMASA 熊本大学, 大学院生命科学研究部, 准教授 (90244128)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 乳酸菌 / 細胞 / リプログラム / 多能性

Outline of Final Research Achievements

Previously, we demonstrated the generation of multipotential cells from adult human dermal fibroblast (HDF) cells by incorporating lactic acid bacteria (LAB) (Ohta et al., PLOS ONE e51866, 2012). LAB-incorporated cell clusters differentiated into endodermal, mesodermal, and ectodermal cells in vivo and in vitro.
Next, we purified the LAB-derived materials from the homogenized LAB through ion-changed and gel-filtration chromatography by observing their cell clusters forming activity. We applied the peak fraction to MALDI-TOF-MS analysis and determined the LAB-derived reprogramming material. When we applied the LAB-derived material to HDF cells, they formed cell clusters and differentiated into cells derived from three germ layers. LAB-derived material was incorporated into the cells by endocytosis and they localized not only in the cytoplasmic region but also in the nucleus．Thus, LAB-derived material reprograms the host cells and modify their gene expression patterns.

Free Research Field

生物学