Establishsment of the efficient gene knock-in technique in the Drosophila genome

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650088
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: NAKAMURA Akira 熊本大学, 発生医学研究所, 教授 (90323245)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ショウジョウバエ / TALEN / CRISPR/Cas9 / 遺伝子ノックアウト / ゲノム編集 / 相同組み換え

Outline of Final Research Achievements

To accelerate artificial manipulations of the Drosophila genome in the post-genome project era, we sough to establish genome editing methodologies in Drosophila using TALEN and CRISPR/Cas9 technologies. We have succeeded in establishing protocols for gene knockout as well as knock-in through homologous recombination. Although gene knock-in efficiencies were varied from a few percent to ~90%, depending on targets, we have already generated GFP knocked-in strains in seven different loci, indicating that the gene knock-in methodology in Drosophila has been practically established.

Free Research Field

発生生物学