2014 Fiscal Year Final Research Report
Establishment of standard cell line in fern plant for the analysis of cell proliferation
| Project/Area Number |
25650091
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 培養細胞 / シダ植物 / プロトプラスト |
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| Outline of Final Research Achievements |
Focusing on the fern plant to make the suspension culture for establishing the standard cell line suitable for analysis of the proliferation. The spores of Ceratopteris richardii were aseptically germinated and transplanted to the gellan gum medium and agar medium of various hormone levels to induce callus. From some good-growing calluses, the suspension cultures were established. The cell lines were stably maintained in a LS liquid medium. Further, the protoplasts were isolated using Cellulase RS and Pectolyase Y23, and then were subsequently cultured. This is a rare study result in fern plants.
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| Free Research Field |
植物細胞生物学
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