2014 Fiscal Year Final Research Report
Molecular properties of signal transducing proteins on the plasma membrane
| Project/Area Number |
25650093
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 細胞内情報変換 / 細胞膜 / カルシウム / ホスファチジルイノシトール / 情報伝達 |
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| Outline of Final Research Achievements |
We investigated molecular properties of the plasma membrane Ca2+-binding proteins, PCaP1 and PCaP2, at biochemical and molecular biological levels. We found that PCaP2 is essential for formation and tip growth of root hairs using loss-of-function mutant lines of PCaP2. Our key finding is that PCaP2 transduces a Ca2+ signal to phosphatidylinositol signal on the plasma membrane at tip of root hair. When the N-terminal peptide of PCaP2 was introduced to plants under the control of CaM 35S promoter, no root hair was observed in the transformant. Using this mutant, we quantified physiological functions of root hair, such as absorption of water and mineral ions. Also we obtained structural information of PCaP1 on the binding to membrane phospholipids and metal ions and found that loss-of-function mutant of PCaP1 has a defect in stomatal closure in the dark.
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| Free Research Field |
生化学
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