Interactome analysis with biotin ligase in Arabidopsis

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650096
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: TAMURA Kentaro 京都大学, 理学(系)研究科(研究院), 助教 (40378609)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: インタラクトーム / ビオチン / ビオチンライゲース / シロイヌナズナ

Outline of Final Research Achievements

Interactomics is a powerful resource to reveal the whole set of molecular protein network in the cells. In this study, we aimed to develop a novel tool for interactomics in plant nuclear envelope by using modified biotin ligase (BirA). We generated transgenic Arabidopsis plants expressing BirA-fused nuclear envelope protein. Western blot analyses indicated that the biotinylated proteins are specifically accumulated in the transgenic plants. However, the accumulation level of biotinylated proteins was low. To purify and identify more effectively, it is required for the system allowing higher accumulation of biotinylated proteins in the transgenic plants

Free Research Field

植物細胞生物