Development and application of a micro-area illumination method for plants

2015 Fiscal Year Final Research Report

• Project/Area Number: 25650098
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: Oyama Tokitaka 京都大学, 理学(系)研究科(研究院), 准教授 (30324396)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 光応答 / ルシフェラーゼ / 概日時計 / 生物発光 / ウキクサ

Outline of Final Research Achievements

We developed a technique that enables a micro-area illumination in a duckweed plant by fixing a tip of an optical fiber inside the plant body. This method should be used for the analysis about cell-to-cell light signal transmission: from light-perceived cells to cells without receiving light. To analyze the cell-to-cell light signal transmission in the circadian clock system, we developed a bioluminescence monitoring system at a single-cell level. This will be used in combination with the micro-area illumination method.

Free Research Field

生物学