Elucidation of molecular analysis for chloroplast genome translocation using versatile model-plant Marchantia

2015 Fiscal Year Final Research Report

• Project/Area Number: 25650126
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Institute of Physical and Chemical Research (2014-2015); Kyoto University (2013)
• Principal Investigator: Ueda Minoru 国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (30632541)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: オルガネラゲノム収奪 / 進化 / 葉緑体 / ゼニゴケ

Outline of Final Research Achievements

Having efficient chloroplast genome transformation and genetic screening system established in Marcantia, measurement of genome translocation rate from chloroplast genome to the nucleus has been done. Analysis of measurement of genome translocation rate revealed that the translocation rate is quite low in Marchantia. Because Marchantia suppresses the translocation, genetic screening using T-DNA insertion lines has been done to find a mutant in which the translocation occurs. As a result, one mutant has been isolated and another allele for the gene was prepared by gene targeting. Now, I am not able to conclude because NGS analysis have not detected any novel chloroplast genome insertion in the nucleus in mutants generated by gene targeting. I am still analyzing while improving method.

Free Research Field

実験進化学