The role of non-S DNA synthesis on the local mutation rate

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650130
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Kyushu University
• Principal Investigator: OHNO Mizuki 九州大学, 医学(系)研究科(研究院), 助教 (70380524)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 分子進化 / 突然変異

Outline of Final Research Achievements

A major source of spontaneous mutations is DNA replication error; therefore, a mutation rate depends on the frequency of DNA replication. However, local mutation rate varies across the genome. It is well known that the non-S DNA syntheses naturally occur in non-S phase cells in vivo. It is speculated that, in the genomic regions having a tendency of non-S DNA synthesis, mutation rate may be increased by the increased frequency of DNA synthesis. The role of non-S DNA synthesis on the local mutation rate was assessed by analyzing the incorporation of BrdU in the pachytene spermatocytes isolated from the mouse testes. Colocalization of BrdU and SCP3 (a maker for synaptonemal complex) immunofluorescence signals was detected in non-S pachytene spermatocytes, suggesting that non-S DNA synthesis detected here descend from a homologous recombination-dependent DNA synthesis.

Free Research Field

分子遺伝学