Development of a quick method to construct linkage maps for genome-scan analysis in non-model plant species

2014 Fiscal Year Final Research Report

• Project/Area Number: 25650140
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Tohoku University
• Principal Investigator: SUYAMA Yoshihisa 東北大学, (連合)農学研究科(研究院), 准教授 (60282315)
• Research Collaborator: MATSUKI Yu ; NAKAZAWA Fumio
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 連鎖解析 / 次世代シーケンサー / 花粉DNA

Outline of Final Research Achievements

We developed a quick method to construct linkage maps with pollen DNA using next-generation sequencer (NGS). As one of the applicable techniques in this study, we developed a PCR-based procedure to construct reduced representation libraries, representing de novo single nucleotide polymorphism (SNP) discovery, and its genotyping using NGS. We then demonstrated the applicability of the method in pollen DNA analysis. Mendelian 1:1 segregation in haploid pollen grains from one individual plant was demonstrated for 226 SNP markers discovered by the method. We concluded that this approach is applicable to construct linkage maps in non-model plant species.

Free Research Field

森林分子生態学